MI-506 and MI-508
Flexible pH Microelectrodes
Flexible pH Electrode along with a
standard laboratory calomel or
silver-silver chloride reference
electrode with liquid junction, can
be used as the electrode pair. The
pair is calibrated in at least two
pH buffer solutions which are close
to the pH values expected. Follow
the procedure recommended by the
manufacturer of your pH meter for
calibrating your pH meter with our
Optimum Response Time:
Optimum response time will be
obtained after the probe has been
exercised in two buffer solutions.
Place a pH 4.01 buffer or equivalent
in a beaker and a pH 6.86 buffer or
equivalent in a second beaker.
the MI-506/MI-508 and reference
electrode together and touch the pH
4.01 buffer allowing 15-20 seconds
for equilibration. Rinse the two
electrodes with distilled water and
then touch the pH 6.86 buffer in the
same manner. Do this several times.
A skin reference (i.e. our A-30) is
applied to the body of the patient
and the pH electrode is placed into
a test tube containing a pH 7 buffer
solution. The patient touches
either the buffer solution or a
buffer soaked gauze extending from
the buffer solution. The pH 7
reading can now be calibrated on the
meter. A second pH electrode is now
placed into this second test tube
and the patient must now make
contact with this buffer as
mentioned above. The pH electrode
can also be calibrated on the
technician before measurements on a
patient. However, this method of
calibration can result in actual pH
error up to 0.5 pH units.
Sterilization and Disinfection
electrode can be sterilized in
ethylene oxide or chemically
sterilized with gluteraldehyde
solutions such as Cidex 7.
Note: Probe cannot be autoclaved.
The electrode can be disinfected
with ethyl alcohol or zephirin
Accepted methods of sterilization
should be followed prior to usage.
Avoid excessive bending or sharp
angle bends just behind the rigid
When using the
microelectrode in solutions
containing protein, it should be
soaked in an enzyme cleaning
solution such as Terg-a-zyme
Inc.) or a chromic
sulfuric glass cleaning solution can
be used to remove the protein build
Note: When cleaning, never
pinch the electrode between fingers
and pull through for drying ---
always pat dry.
After cleaning, rinse thoroughly
with distilled water.
Always clean the microelectrode
Long-term (over 2 weeks):
over 2 weeks): Return the electrode
to its original container and
prepare it in the same condition in
which you received it.
probe can be left in an acid pH
buffer solution (pH 4.01).