Dip-type Reference Microelectrodes
The Reference Electrode Kit contains
1 Bottle of
1 Set of
Reference microelectrodes can be
used with any pH, ion-selective or
redox electrode. The electrode is
composed of an internal
silver-silver chloride electrode
with an internal filling solution of
3 M KCl saturated with AgCl. The
junction is either a silicate or
ceramic frit sealed in glass. Before
the electrode can be placed into
operation, the reference barrel must
be filled with the internal
Filling the Reference Barrel
The barrel is removed from the
electrode cap by unscrewing it.
The 3M KCl solution is added to
the barrel with the filling tube
attached to the nipple of the
bottle. Fill the barrel within
one inch from the top.
The Ag-AgCl wire, which is
attached to the electrode cap,
is then inserted into the barrel
and locked in place.
When using the electrode in
solutions containing protein, the
electrode should be soaked in an
enzyme cleaning solution such as
Inc.) for 10 -15 seconds to
remove protein from the reference
junction. This will prolong the
useful life of the electrode.
Long-term (over 4 weeks):
Remove the barrel containing the
electrolyte and store the entire
barrel in a stoppered test tube
filled with reference electrolyte.
Rinse the silver wire and electrode
cap to remove the salt solution and
dry using an absorbent towel. Store
the electrode in its original box or
any closed container to keep the
dust off of the electrode.
Place the tip of the electrode into
a test tube or beaker containing